BD Pharmingen- Biotin Rat Anti-Mouse V- 6 T-Cell Receptor_BD Pharmingen
产品信息
抗原名称
TCR Vβ6
宿主
Rat F344, also known as Fischer, CDF IgG2b, λ
免疫原
C57BL/6 Mouse Helper T-Cell Clone OI11
简单描述
The RR4-7 antibody specifically reacts with the Vβ 6 T-Cell Receptor (TCR) of mice having the
a
(
e.g.,
C57BR, C57L, SJL) and
b
(
e.g.,
A, BALB/c, CBA/Ca, C3H/He, C57BL, DBA/1) haplotypes of the
Tcrb
gene complex. The
Tcrb-V6
gene locus is deleted in mice having the
c
(
e.g.,
RIII) haplotype. Vβ 6 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen encoded by
Mtv-7
(
Mls-1[a], Mls[a]
) endogenous provirus (
e.g.,
AKR, CBA/J, C58, DBA/2, NZB), or
Mtv
-43 endogenous provirus (
e.g.,
MA/MyJ). Exogenous MMTV-SW, as well as endogenous
Mtv
-44-encoded superantigen (
e.g.,
NZW), also causes incomplete elimination of Vβ 6 TCR-expressing T cells. Plate-bound RR4-7 antibody activates Vβ 6 TCR-bearing T cells, soluble RR4-7 mAb blocks
in vitro
proliferation and cytolytic activities of Vβ 6 TCR-bearing T-cell clones, and injection of the antibody results in in vivo depletion of Vβ 6 TCR-bearing T cells.
商品描述
RR4-7
The RR4-7 antibody specifically reacts with the Vβ 6 T-Cell Receptor (TCR) of mice having the
a
(
e.g.,
C57BR, C57L, SJL) and
b
(
e.g.,
A, BALB/c, CBA/Ca, C3H/He, C57BL, DBA/1) haplotypes of the
Tcrb
gene complex. The
Tcrb-V6
gene locus is deleted in mice having the
c
(
e.g.,
RIII) haplotype. Vβ 6 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen encoded by
Mtv-7
(
Mls-1[a], Mls[a]
) endogenous provirus (
e.g.,
AKR, CBA/J, C58, DBA/2, NZB), or
Mtv
-43 endogenous provirus (
e.g.,
MA/MyJ). Exogenous MMTV-SW, as well as endogenous
Mtv
-44-encoded superantigen (
e.g.,
NZW), also causes incomplete elimination of Vβ 6 TCR-expressing T cells. Plate-bound RR4-7 antibody activates Vβ 6 TCR-bearing T cells, soluble RR4-7 mAb blocks
in vitro
proliferation and cytolytic activities of Vβ 6 TCR-bearing T-cell clones, and injection of the antibody results in in vivo depletion of Vβ 6 TCR-bearing T cells.
同种型
Rat F344, also known as Fischer, CDF IgG2b, λ
克隆号
克隆 RR4-7 (RUO)
浓度
0.5 mg/ml
产品详情
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
TCR Vβ6
背景
别名
TCR V beta 6; TCR Vβ6
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(14)
1. Fairchild S, Rosenwasser OA, Dyson PJ, Tomonari K. Tcrb-V3+ T-cell deletion and a new mouse mammary tumor provirus, Mtv-44. Immunogenetics. 1992; 36(3):189-194. (Biology).
2. Haqqi TM, Banerjee S, Anderson GD, David CS. RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes. J Exp Med. 1989; 169(6):1903-1909. (Biology).
3. Held W, Shakhov AN, Waanders G, et al. An exogenous mouse mammary tumor virus with properties of Mls-1a (Mtv-7). J Exp Med. 1992; 175(6):1623-1633. (Biology).
4. Jones LA, Chin LT, Longo DL, Kruisbeek AM. Peripheral clonal elimination of functional T cells. Science. 1990; 250(4988):1726-1729. (Biology).
5. Jones LA, Chin LT, Merriam GR, Nelson LM, Kruisbeck AM. Failure of clonal deletion in neonatally thymectomized mice: tolerance is preserved through clonal anergy. J Exp Med. 1990; 172(5):1277-1285. (Biology).
6. Kanagawa O, Palmer E, Bill J. The T cell receptor V beta 6 domain imparts reactivity to the Mls-1a antigen. Cell Immunol. 1989; 119(2):412-426. (Immunogen: Blocking).
7. Kanagawa O. In vivo T cell tumor therapy with monoclonal antibody directed to the V beta chain of T cell antigen receptor. J Exp Med. 1989; 170(5):1513-1519. (Clone-specific: Depletion).
8. Kruisbeek AM, Shevach EM. Proliferative assays for T cell function. Curr Protoc Immunol. 2004; 3:3.12.1-3.12.14. (Clone-specific: Activation).
9. Ramsdell F, Lantz T, Fowlkes BJ. A nondeletional mechanism of thymic self tolerance. Science. 1989; 246(4933):1038-1041. (Biology).
10. Rocha B, Vassalli P, Guy-Grand D. The V beta repertoire of mouse gut homodimeric alpha CD8+ intraepithelial T cell receptor alpha/beta + lymphocytes reveals a major extrathymic pathway of T cell differentiation. J Exp Med. 1991; 173(2):483-486. (Biology).
11. Rudy CK, Kraus E, Palmer E, Huber BT. Mls-1-like superantigen in the MA/MyJ mouse is encoded by a new mammary tumor provirus that is distinct from Mtv-7. J Exp Med. 1992; 175(6):1613-1621. (Biology).
12. Tomonari K, Fairchild S. Positive and negative selection of Tcrb-V6+ T cells. Immunogenetics. 1992; 36(4):230-237. (Biology).
13. Utsunomiya Y, Kosaka H, Kanagawa O. Differential reactivity of V beta 9 T cells to minor lymphocyte stimulating antigen in vitro and in vivo. Eur J Immunol. 1991; 21(4):1007-1011. (Clone-specific: Activation).
14. Webb S, Morris C, Sprent J. Extrathymic tolerance of mature T cells: clonal elimination as a consequence of immunity. Cell. 1990; 63(6):1249-1256. (Biology).
数据库链接
Entrez-Gene ID
110317
参考图片
Two-color analysis of the expression of Vβ 6 TCR on peripheral T lymphocytes. C57BL/6 lymph nodes cells were incubated simultaneously with Biotin Rat Anti-Mouse Vβ 6 T-Cell Receptor (Cat. No. 553192; right panel), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033) monoclonal antibodies, followed by Avidin FITC (Cat. No. 554057). The fluorescence contour plots were derived from gated events based on the forward and side light-scattering of viable lymphocytes. Flow cytometry was performed on a FACScan™.
Two-color analysis of the expression of Vβ 6 TCR on peripheral T lymphocytes. C57BL/6 lymph nodes cells were incubated simultaneously with Biotin Rat Anti-Mouse Vβ 6 T-Cell Receptor (Cat. No. 553192; right panel), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033) monoclonal antibodies, followed by Avidin FITC (Cat. No. 554057). The fluorescence contour plots were derived from gated events based on the forward and side light-scattering of viable lymphocytes. Flow cytometry was performed on a FACScan™.
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